CRISPR Cas9 Workshop




Pankaj  Dr. Pankaj Bhowmik graduated with honors from Bangladesh Agricultural University and joined at the Bangladesh Agricultural Research Institute as a Scientific Officer (Wheat Breeding). He received his Ph.D. in Plant Physiology and Molecular Biology from Kagawa University, Japan. Following JSPS, Killam and NSERC post-doctoral training at the National Agricultural Research Center in Hokkaido, University of Alberta and Agriculture and Agri-Food Canada, Dr. Bhowmik joined the National Research Council of Canada in Saskatoon, where he is now a Research Officer and a member of the Cell Technologies team. Dr. Bhowmik with over 15 years of experience specializes in tissue culture, plant transformation and gene editing.  Since 2009 after joining NRC he has been working on microspore transformation and plant regeneration of canola and wheat combining doubled haploidy and genetic engineering tools (CRISPR). Dr. Bhowmik is in the process of developing a high throughput gene editing platform in collaboration with other NRC Labs, GIFS and Thermo Fisher Scientific. In a collaborative research project funded by Thermo Fisher, Dr. Bhowmik has successfully used and optimized the Neon transfection system to deliver Cas9-dsRed plasmid DNA into wheat microspores. Pankaj is currently working on the Canadian Wheat Improvement program and also part of the team working on the ADF funded project (# 20150358; PI – Dr. Manoj Kulkarni) to develop male sterile lines in wheat using genome editing tools. In collaboration with Dr. Bunyamin T’aran at the University of Saskatchewan, Dr. Bhowmik has also been working on a project funded by SaskPulse for proof of concept validation of CRISPR- based gene editing tools in Chickpea. 
Yurdagul Ferhatoglu Yurdagul Ferhatoglu is the co-recipient of SASKPULSE Proof of Concept Grant aimed to use CRISPR based gene editing tools in the development of metribuzin tolerant chickpea at the Plant Science Department of U. of Saskatchewan. Previously, Ferhatoglu has worked at the University of Illinois, Urbana-Champaign on functional genomics of Arabidopsis P450s and at NRC-PBI, Saskatoon, she has worked on drought resistant GMO canola development; at Phenomenome Discoveries, she has overseen plant metabolomic projects, providing insight into gene mutations and biological processes. Ferhatoglu was also part of the team discovering chloroplastic gene targeting by the herbicide clomazone at U of Kentucky and function of mrg15, an MSL complex protein, an epigenetic modulator in gene regulation. In past, she served as senate member at U of Saskatchewan and advocate for water and climate friendly campaigns at Saskatchewan Environmental Society.
Brett Dalman

Brett Dalman is a Senior Account Manager for Thermo Fisher Scientific (TFS). She worked at the Canadian Food Inspection Agency for 4 years and has been working in the science sales field for 8 years. She prides herself in supporting her customer base and assisting with their experimental designs. She works with numerous groups across the TFS organization to enable her customers to have the best experience possible. By leveraging her science background and knowledge she provides unique and diverse solutions. 

 
Udhaya Kannan

Dr. Udhaya Kannan is a postdoctoral fellow at the University of Saskatchewan with Dr. Curtis Pozniak, Department of Plant Sciences, and Dr. Kevin Rozwadowski, Agriculture and Agri Food Canada, Saskatoon. She was born in Trichy, Tamil Nadu, India and completed her bachelor’s degree in biotechnology at Chennai, India. She then pursued her master’s degree at Newcastle University, Newcastle upon Tyne, UK and PhD from the Department of Plant Sciences, University of Saskatchewan. Her PhD work focussed on biochemical and molecular characterization of raffinose family oligosaccharide biosynthesis in lentil. Her current project focuses on developing wheat lines with directed alteration of recombination processes by manipulating gene expression by CRISPRi and genetic characterization of these lines for modulated meiotic recombination frequency. 

Tawhid received a Bachelor degree in Biotechnology from Khulna University in Bangladesh. He completed a Master’s degree in Bioprocess Technology from Asian Institute of Technology (AIT) in Thailand. He came to Canada with the intention to be trained up as a molecular biologist, and became a Doctoral student at the Biology department of University of Western Ontario, where he completed a Ph.D. degree under the guidance of Dr. Priti Krishna. He received an NSERC Visiting Research Fellowship for post-doctoral study at the Saskatoon Research Center of Agriculture and Agri-Food Canada with Dr. Kevin Rozwadowski.  He is now working with Dr. Karen Tanino at Plant Sciences department of University of Saskatchewan as a Research Officer. His current research is focusing on understanding the chilling and frost stress tolerance in corn. He is aiming to apply CRISPR-Cas9 mediated genome editing to engineer the key ultra-structural and physiological phenotypes that can increase the cold and drought stress resistance in corn.

Kevin R

Kevin Rozwadowski is a Research Scientist at the Saskatoon Research Centre of Agriculture and Agri-Food Canada.  Kevin received a B.Sc. in Agriculture from the University of Manitoba (1987), a M.Sc. in Applied Microbiology from the University of Saskatchewan (1990) and a Ph.D. in Molecular Biology and Genetics from the University of Guelph (1996).  His areas of research include: DNA recombination and repair in plants, and mechanisms of gene and protein regulation.  This work is focused on developing technologies to modulate meiotic recombination frequency to expedite plant breeding programs, to facilitate efficient gene editing in plants, and to regulate expression of large gene families in polyploid crops.

Sateesh

Sateesh Kagale is a Research Officer at the National Research Council of Canada in Saskatoon. He received a B.Sc. in Agriculture from The University of Agricultural Sciences Dharwad and a M.Sc. in Plant Pathology from Tamil Nadu Agricultural University Coimbatore in India. He then moved to Canada to pursue doctoral studies in Cell and Molecular Biology at the University of Western Ontario. Sateesh has more than 10 years of research experience in applied crop genomics and biotechnology. His current research focuses on application of genome analysis and editing technologies to wheat improvement. 

Abidur Rahman

Dr. Abidur Rahman completed his Ph.D. from Kobe University, Japan in 2001, and postdoctoral studies from University of Massachusetts, Amherst, USA. He is currently working as an Associate Professor in the Faculty of Agriculture, Iwate University, Japan. His scientific work is centered on understanding the role of plant growth hormones, especially auxin and ethylene, in regulating plant growth and development at both optimal and stressed conditions. His group is also working on phytoremediation, genome wide association (GWAS), and high throughput genome sequencing. He and his research group published more than 30 outstanding articles in high impact plant journals like The Plant Cell, Plant Physiology, The Plant Journal etc.

Besides his research and academic activities, Dr. Rahman is serving in the editorial board of PLOS ONE, Journal of Plant Growth Regulation and Frontiers in Plant Science. He also serves as a reviewer for grant applications from NSF, ISF, BARD, FWF, BSF. Dr Rahman is also currently serving as the head of the International affairs section of the United Graduate School of Agricultural Sciences (UGAS), Iwate University, Morioka, Japan.

Xiaomu Xiaomu received his B.S. and M.S. degrees from Peking University in China, before he came to the U.S. for his doctoral training at Purdue University, where he received a Ph.D in Plant Molecular Biology, under the direction of Dr. Paul M Hasegawa.  He went on to become an Ag biotechnologist, first through a postdoc work on genetic engineering of mint and sorghum crops, and then joining Pioneer in 1998 to work with biotechnology traits in corn.  There he has worked on various trait discovery and product development projects.  He is currently a research manager at DuPont Pioneer, leading a team of scientists to apply CRISPR-Cas9 genome editing technology in trait development.


Symposium

Over 20 speakers from the four participating universities (UGAS, Iwate University, University of Dhaka, Fujian Agriculture and Forestry University, and University of Saskatchewan) have been invited. The lecture sessions will be held 9 am - noon and 3 - 5 pm. 

Bueckert Rosalind A. Bueckert, Department of Plant Sciences, University of Saskatchewan
Supratim Ghosh Supratim Ghosh, Department of Food and Bioproduct Sciences, University of Saskatchewan
Zabed Hossain  Mohammed Zabed Hossain, Department of Biology, University of Dhaka
Mohammad Nurul Islam   Mohhamed Nurul Islam, Department of Biology, University of Dhaka 
Miura Makoto Makoto Miura, United Graduate School of Agricultural Sciences, and Department of Biological Chemistry and Food Sciences, Iwate University
miyazakinasao Masao Miyazaki, United Graduate School of Agricultural Sciences, and Department of Biological Chemistry and Food Sciences, Iwate University
Akira Nakajima Akira Nakajima, United Graduate School of Agricultural Sciences, Iwate University and Department of Applied Biology and Food Sciences, Hirosaki University
Nickerson Michael Nickerson, Department of Food and Bioproduct Sciences, University of Saskatchewan
Yuan Qin Yuan Qi, Center for Genomics and Biotechnology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University
Abidur Rahman

Abidur Rahman, United Graduate School of Agricultural Sciences, and Cryobiofronteer Research Center, Iwate University

Michiko Sasabe Michiko Sasabe, United Graduate School of Agricultural Sciences, Iwate University and and Department of Biology, Hirosaki University
Tune Sasanuma Tsuneo Sasanuma, United Graduate School of Agricultural Sciences, Iwate University and Department of Food and Applied Life Sciences, Yamagata University
Zeba Sarah Zeba Islam Seraj, Department of Biochemistry and Molecular Biology, University of Dhaka
Sharbel Tim Sharbel, Department of Plant Sciences, University of Saskatchewan
Shirtliffe

Steve Shirtliffe, Department of Plant Sciences, University of Saskatchewan

Yoshihito Shiono, United Graduate School of Agricultural Sciences, Iwate University and Department of Food and Applied Life Sciences, Yamagata University

Vladimir Vujanovic Vladimir Vujanovic, Department of Food and Bioproduct Sciences, University of Saskatchewan
Hong Wang Hong Wang, Department of Biochemistry, University of Saskatchewan
Suang     Shuang Wu, Horticultural Plant Biology and Metabolomics Center, Fujian Agriculture and Forestry University
Miwa

Miwa Yamada, United Graduate School of Agricultural Sciences, and Department of Biological Chemistry and Food Sciences, Iwate University